公司简介

北京西浓科技有限公司成立于 2014 年，注册于北京市中关村高科技园区，专注于各种实验动物的脑缺血模型的技术开发、技术咨询和相关产品的生产、销售，致力于为广大客户提供优质的与 MCAO 模型相关的一站式服务。创始人具有雄厚的猴、狗、兔、大鼠、小鼠等实验动物的模型制备技术，其中对大鼠/小鼠的大脑中动脉线栓模型（MCAO 模型）具有 20 多年的制备经验，研发的主要产品 MCAO 线栓已上市销售近 20 年，已被美国、加拿大、香港、中国大陆等的科研机构、大学、医院广泛采用，近百篇研究论文发表于国内外数十种优秀专业期刊 (部分论文见附录)，丁香通点击量高达 56123 次（截至 2018.10.22）。

MCAO 线栓业务以前由北京沙东⽣物技术有限公司经营，从 2014 年开始将由西浓科技独立接管经营与MCAO 业务相关的研发、生产、销售及售后服务，之前的联系电话、邮箱等不变，并启用全国统一的服务热线 400-863-6007, 公司官网为 www.mcao-model.com.cn。请认准公司商标「西浓科技/CINONTECH」，这是我们对优质产品及技术服务的承诺！

MCAO 栓线

国内最早实行标准化、规模化、商品化的 MCAO 栓线（以发表论文数量为证）。MCAO 栓线（MCAO Monofilaments）是制备大鼠/小鼠（或其它实验动物）局部脑缺血模型的非常关键的实验材料，本产品是用柔韧适度的单丝尼龙线，经显微操作烧制而成，其头端光滑、大小一致，易进入颅内又不至于刺破血管，使用本产品可大大提高模型制备的成功率，及脑缺血范围的稳定性（这段话的原始出处是我们的产品介绍，但目前已经被多家公司剽窃使用）。MCAO 栓线，我们有最多最全的种类（4 大类）：硅胶包被栓线、无包被双球栓线、多聚赖氨酸包被栓线、无包被单球栓线；最多的级别（6 个级别）：A1、A2、A3、A4、A5、A6，分别满足不同研究目或经济条件的需要。国内外其他厂家目前只是单一品种（硅胶包被栓线）。

各类栓线特点：

硅胶包被栓线：在尼龙线头端 2-5 mm 的一段包被硅橡胶，硅胶直径与动物体重相匹配，一般用较细的尼龙线制作（4-0、5-0）。

无包被双球栓线：本公司独家产品，在尼龙线前端间隔 2-5 mm 分别采用特殊工艺烧制两个与动物体重相匹配的球状物，无任何包被，一般用较细的尼龙线制作（4-0、5-0）。

无包被单球栓线：本公司独家产品，根据动物体重匹配使用不同直径的尼龙线（如 2-0、3-0、4-0），并将尼龙线顶端烧制成一个与动物体重相匹配的圆球形，无任何包被。

多聚赖氨酸包被栓线：本公司独家产品，根据动物体重匹配使用不同直径的尼龙线（如 2-0、3-0、4-0），并将尼龙线顶端烧制成一个与动物体重相匹配的圆球形，栓线经过 0.1% 多聚赖氨酸的包被处理。

各类栓线的优缺点：

硅胶栓线：成本较高，梗死面积相对大而稳定，模型症状较重，适于短时间的急性期观察研究、不适于长时间的缺血或永久性缺血研究；硅胶易脱落不便消毒和多次重复使用；硅胶成份复杂，组织相容性差，刺激血管内皮。栓线较细、较软，但可降低蛛网膜下腔出血概率。

无包被双球栓线：成本略高，梗死面积相对大而稳定，模型症状较重、适于短时间的急性期观察研究、不适于长时间的缺血或永久性缺血研究；纯尼龙材料、无包被，组织相容性好，可多次重复使用。栓线较细、较软，但可降低蛛网膜下腔出血概率。

无包被的单球栓线：成本较低，梗死面积相对小，模型症状较轻、适于长时间的缺血或永久性缺血研究；纯尼龙材料、无包被，组织相容性好，便于消毒可多次重复使用。栓线相对较粗、较硬，但对初学者可增加蛛网膜下腔出血概率。

赖氨酸包被的栓线：成本适中，梗死面积及稳定性与硅胶栓线和双球栓线相似，模型症状较重、但多聚赖氨酸可能会刺激血管内皮，也不便多次重复使用。栓线相对较粗、较硬，但对初学者可增加蛛网膜下腔出血概率。

六个级别介绍：

产品分六个级别，每个级别分若干型号和规格。

A1 级：无包被的单球栓线，无标记、未消毒，需要用户自己消毒、做标记；

A2 级：无包被的单球栓线，有标记*、已消毒，可以直接使用；

A3 级：多聚赖氨酸包被栓线#、无标记、已消毒，需要用户自己做标记；

A4 级：多聚赖氨酸包被栓线，有标记、已消毒，即买即用型，不需任何处理。

A5 级：硅胶包被栓线，有标记、已消毒，即买即用型，不需任何处理。

A6 级：无包被双球栓线，无包被、有标记*、已消毒，可以直接使用。

大鼠线栓的标记在距头端 19-20 mm 处，小鼠线栓的标记在距头端 9-10 mm 处.

# 前端 20 mm(大鼠线栓) 或 10 mm(小鼠线栓) 包被多聚-L-赖氨酸（包被后不会改变栓线的直经）。栓线包被多聚-L-赖氨酸可以提高 MCAO 模型的稳定性。
 

A1/A2/A3/A4 栓线

A5 栓线

A6 栓线

使用本公司 MCAO 栓线发表的部分英文论文

（论文中检索到的「Beijing Cinontech Co. Ltd.」或「Beijing Sunbio Biotech Co. Ltd.」都是指使用本公司栓线产品）

1. Dong B, et al. Hemopexin alleviates cognitive dysfunction after focal cerebral ischemia-reperfusion injury in rats. BMC Anesthesiol. 2019; 19: 13. Published online 2019 Jan 15. doi: 10.1186/s12871-019-0681-2.

2. He H, et al. Neuronal autophagy aggravates microglial inflammatory injury by downregulating CX3CL1/fractalkine after ischemic stroke. Neural Regen Res. 2019 Feb; 14(2): 280–288.

3. Ran Y, et al. Splenectomy Fails to Provide Long-Term Protection Against Ischemic Stroke. Aging Dis. 2018 Jun; 9(3): 467–479.

4. Li X, et al. Intranasal administration of nerve growth factor promotes angiogenesis via activation of PI3K/Akt signaling following cerebral infarction in rats. Am J Transl Res. 2018; 10(11): 3481–3492.

5. Lin X, et al. iTRAQ-Based Proteomics Analysis Reveals the Effect of Rhubarb in Rats with Ischemic Stroke. Biomed Res Int. 2018; 2018: 6920213.

6. Xu F, et al. Brain-Derived Glia Maturation Factor β Participates in Lung Injury Induced by Acute Cerebral Ischemia by Increasing ROS in Endothelial Cells. Neurosci Bull. 2018 Dec; 34(6): 1077–1090.

7. Bi M, et al. Bone mesenchymal stem cells transplantation combined with mild hypothermia improves the prognosis of cerebral ischemia in rat. PLoS One. 2018; 13(8): e0197405.

8. Qu B, et al. The Antiapoptosis Effect of Geum japonicum Thunb. var. chinense Extracts on Cerebral Ischemia Reperfusion Injury via PI3K/Akt Pathway. Evid Based Complement Alternat Med. 2018; 2018: 7290170.

9. Zheng X, et al. Buyang Huanwu Decoction Targets SIRT1/VEGF Pathway to Promote Angiogenesis After Cerebral Ischemia/Reperfusion Injury. Front Neurosci. 2018; 12: 911.

10. Yao G, et al. Ischemic postconditioning confers cerebroprotection by stabilizing VDACs after brain ischemia. Cell Death Dis. 2018 Oct; 9(10): 1033.

11. Gong Z, et al. Mitochondrial dysfunction induces NLRP3 inflammasome activation during cerebral ischemia/reperfusion injury. J Neuroinflammation. 2018; 15: 242.

12. Wang Z, et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci. 2017; 10: 358.

13. Guo C, et al. The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury. Sci Rep. 2017; 7: 3039.

14. Guo C, et al. The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury. Sci Rep. 2017; 7: 3039.

15. Wu R, et al. TREM2 protects against cerebral ischemia/reperfusion injury. Mol Brain. 2017; 10: 20.

16. Yu HY, et al. Comparison of the glycopattern alterations of mitochondrial proteins in cerebral cortex between rat Alzheimer’s disease and the cerebral ischemia model. Sci Rep. 2017; 7: 39948.

17. Zhang Q, et al. Optimization of Huang-Lian-Jie-Du-Decoction for Ischemic Stroke Treatment and Mechanistic Study by Metabolomic Profiling and Network Analysis. Front Pharmacol. 2017; 8: 165.

18. Zhang Q, et al. Treatment Effects of Ischemic Stroke by Berberine, Baicalin, and Jasminoidin from Huang-Lian-Jie-Du-Decoction (HLJDD) Explored by an Integrated Metabolomics Approach. Oxid Med Cell Longev. 2017; 2017: 9848594. Published online 2017 Aug 14. doi: 10.1155/2017/9848594.

19. Zhang XH, et al. Danshen-Chuanxiong-Honghua Ameliorates Cerebral Impairment and Improves Spatial Cognitive Deficits after Transient Focal Ischemia and Identification of Active Compounds. Front Pharmacol. 2017; 8: 452.

20. Liu XM, et al. Kudiezi injection mitigates myocardial injury induced by acute cerebral ischemia in rats. BMC Complement Altern Med. 2017; 17: 8.

21. Hu Y, et al. Microarray expression profiles of genes in lung tissues of rats subjected to focal cerebral ischemia-induced lung injury following bone marrow-derived mesenchymal stem cell transplantation. Int J Mol Med. 2017 Jan; 39(1): 57–70.

22. Yao YG, et al. MG53 permeates through blood-brain barrier to protect ischemic brain injury. Oncotarget. 2016 Apr 19; 7(16): 22474–22485.

23. Li YT, et al. Pharmacokinetic Comparison of Scutellarin and Paeoniflorin in Sham-Operated and Middle Cerebral Artery Occlusion Ischemia and Reperfusion Injury Rats after Intravenous Administration of Xin-Shao Formula. Molecules 2016, 21(9), 1191.

24. Min XL, et al. MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury? Neural Regen Res. 2015 Nov; 10(11): 1799–1808.

25. Lin J, Zheng C, Zhang X, et al. Effects of Tetramethylpyrazine on Functional Recovery and Neuronal Dendritic Plasticity after Experimental Stroke. Evidence-Based Complementary and Alternative Medicine. Volume 2015, Article ID 394926, 10 pages.

26. Lian T, Qu D, Zhao X, et al. Identification of Site-Specific Stroke Biomarker Candidates by Laser Capture Microdissection and Labeled Reference Peptide. Int. J. Mol. Sci. 2015, 16, 13427-13441.

27. Lin M, Sun W, Gong W, et al. Methylophiopogonanone A Protects againstCerebral Ischemia/Reperfusion Injury and Attenuates Blood-Brain BarrierDisruption In Vitro. PLOS ONE, April 21, 2015.

28. Zhou F, Gao S, Wang L, et al. Human adipose-derived stem cells partially rescuethe stroke syndromes by promoting spatial learning and memory in mousemiddle cerebral artery occlusion model. Stem Cell Research & Therapy (2015)6:92.

29. Wen Y, Zhang X, Dong L, et al. Acetylbritannilactone ModulatesMicroRNA-155-Mediated Inflammatory Response in Ischemic Cerebral Tissues.Mol Med. 2015; 21(1): 197–209.

30. Yao H, Gao M, Ma J, et al. Transdifferentiation-Induced Neural Stem CellsPromote Recovery of Middle Cerebral Artery Stroke Rats. PLoS One. 2015;10(9): e0137211.

31. Fang L, Gao H, Zhang W, et al. Resveratrol alleviates nerve injury after cerebralischemia and reperfusion in mice by inhibiting inflammation and apoptosis.Int J Clin Exp Med. 2015; 8(3): 3219–3226.

32. Hua K, Sheng X, Li TT, et al. The edaravone and 3-n-butylphthalidering-opening derivative 10b effectively attenuates cerebral ischemia injury inrats. Acta Pharmacol Sin. 2015 Aug; 36(8): 917–927.

33. Lan Z, Xu X, Xu W, et al. Discovery of 3-n-butyl-2,3-dihydro-1 H-isoindol-1-oneas a potential anti-ischemic stroke agent. Drug Des Devel Ther. 2015; 9: 3377–3391.

34. Li X, Li C, Wei LY, et al. Hydrogen sulfide intervention in focal cerebralischemia/reperfusion injury in rats. Neural Regen Res. 2015 Jun; 10(6): 932–937.

35. Jiang M, Li J, Peng Q, et al . Neuroprotective effects of bilobalide on cerebralischemia and reperfusion injury are associated with inhibition ofpro-inflammatory mediator production and down-regulation of JNK1/2 andp38 MAPK activation. J Neuroinflammation. 2014; 11: 167.

36. Feng R, et al. Pre-ischemic exercise alleviates oxidative damage followingischemic stroke in rats. Exp Ther Med. 2014 Oct; 8(4): 1325–1329.

37. Zhao G, et al. Pinocembrin Protects the Brain against Ischemia-ReperfusionInjury and Reverses the Autophagy Dysfunction in the Penumbra Area.Molecules. 2014 Oct; 19(10): 15786–15798.

38. Nie B, et al. A Statistical Parametric Mapping Toolbox Used for Voxel-WiseAnalysis of FDG-PET Images of Rat Brain. PLoS One. 2014; 9(9): e108295.

39. Wang Z, et al. PET Demonstrates Functional Recovery after Treatment byDanhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury.Evid Based Complement Alternat Med. 2014; 2014: 430757.

40. Yu S, et al. An Active Component of Achyranthes bidentataPolypeptidesProvides Neuroprotection through Inhibition of Mitochondrial-DependentApoptotic Pathway in Cultured Neurons and in Animal Models of CerebralIschemia. PLoS One. 2014; 9(10): e109923.

41. Sun J, et al. Gradually Increased Training Intensity Benefits RehabilitationOutcome after Stroke by BDNF Upregulation and Stress Suppression. BiomedRes Int. 2014; 2014: 925762.

42. Li H, et al. Isoflurane postconditioning reduces ischemia-induced nuclearfactor-κB activation and interleukin 1β production to provideneuroprotection in rats and mice. Neurobiol Dis. 2013 Jun; 54: 216–224.

43. Zhang N, et al. Chrysophanol Inhibits NALP3 Inflammasome Activation andAmeliorates Cerebral Ischemia/Reperfusion in Mice. Mediators Inflamm.2014; 2014: 370530.

44. Luo Y, Yang YP, Li WH, et al. Neuroprotective effects of madecassoside against focal cerebral ischemia reperfusion injury in rats. Brain Research. Available online 13 April 2014.

45. Wang ZX, Huang WQ, Zuo ZY. Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat. Journal of Neuroinflammation 2014, 11:56.

46. Sun L, Qiang R, Yang Y, et al. L-Serine Treatment May Improve Neurorestoration of Rats after Permanent Focal Cerebral Ischemia Potentially Through Improvement of Neurorepair. PLoS ONE 2014, 9(3): e93405. doi: 10.1371/journal.pone.009340.

47. Li L, et al. Glutamate transporter type 3 mediates isoflurane preconditioning-induced acute phase of neuroprotection in mice. Brain Res Bull.2013 Sep; 98: 23–29.

48. Zhu X, et al. Electroacupuncture preconditioning-induced neuroprotection may be mediated by glutamate transporter type 2. Neurochem Int. 2013 Oct; 63(4):302–308.

49. Lin Y, Zhang JC, Fu J, et al. Hyperforin attenuates brain damage induced by transient middle cerebral artery occlusion (MCAO) in rats via inhibition of TRPC6 channels degradation. Journal of Cerebral Blood Flow & Metabolism (2013) 33, 253–262;

50. An S, Kuang YY, Shen T, et al. Brain-targeting delivery for RNAi neuroprotection against cerebral ischemia reperfusion injury. Biomaterials 34 (2013) 8949-8959.

51. Fu J, Xue R, Gu JF, et al. Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus. Molecular Medicine Reports. 2013, 1708-1714.

52. Cheng FF, Lu Y, Zhong XG. Baicalin's Therapeutic Time Window of Neuroprotection during Transient Focal Cerebral Ischemia and Its Antioxidative Effects In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine, vol.2013, PP.1-11, 2013.

53. Chen XY, Sun XC, Su HX. Upregulation of myeloid cell leukemia-1 potentially modulates beclin-1-dependent autophagy in ischemic stroke in rats. BMC Neuroscience, 2013, 14:56.

54. Dong BB, Cai M, Fang ZP. Hemopexin induces neuroprotection in the ratsubjected to focal cerebral ischemia. BMC Neuroscience 2013, 14:58.

55. Lan R, Xiang J, Zhang Y. PI3K/Akt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine, Volume 2013.

56. Li JJ, Sheng WL, Feng CZ. Pyrrolidine dithiocarbamate attenuates brain Aβincrease and improves long-term neurological outcome in rats after transient focal brain ischemia. Neurobiol Dis. 2012; 45(1): 564–572.

57. Guo JY, Ding J, Yuan F. Dose-Dependent Protective Effect of Bisperoxovanadium against Acute Cerebral Ischemia in a Rat Model of Ischemia/Reperfusion Injury. Int. J. Mol. Sci. 2013, 14, 12013-12022. 

58. Li LL, Zuo ZY. Isoflurane postconditioning induces neuroprotection via Akt activation and attenuation of increased mitochondrial membrane permeability. Neuroscience. 2011 December 29; 199: 44–50.

59. Li H, Yin JB, Li LL, et al. Isoflurance postconditioning reduces ischemia-induced nuclear factor-kB activation and interleukin 1β production to provide neuroprotection in rats and mice. Neurobiology of Disease. Available online 8 January 2013.

60. Zhu SP, Li Y, Lu HY, et al. Imaging the Early Cerebral Blood Flow Changes in Rat Middle Cerebral Artery Occlusion Stroke Model. 34th Annual International Conference of the IEEE EMBS San Diego, California USA, 28 August - 1 September, 2012.

61. Cheng FF, Zhong XG, Lu Y, et al. Refined Qingkailing ProtectsMCAOMice fromEndoplasmic Reticulum Stress-Induced Apoptosis with a Broad Time Window. Evidence-Based Complementary and Alternative Medicine. 2012, Article ID 567872, 12 pages.

62. Zhai FG, Zhang XP, Guan Y, et al. Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats. Neural Regen Res. 2012; 7(12):917-923.

63. Li LL, Zuo ZY. Glutamate transporter type 3 knockout reduces brain tolerance to focal brain ischemia in mice. Journal of Cerebral Blood Flow & Metabolism . 2011; 31:1283-1292. 

64. Ding H, Yan C-Z, Shi H, Zhao Y-S, Chang S-Y, et al. Hepcidin Is Involved in Iron Regulation in the Ischemic Brain. PLoS ONE.2011, 6(9): e25324.

65. Han QQ, Li B, Feng H, et al. The promotion of cerebral ischemia recovery in rats by laminin-binding BDNF. Biomaterials. 2011; 32(22):5077-5085.

66. Xu HY, Zhang CW, Zhang CX. Effect of propofol pretreatment on apoptosis in ratbrain cortex after focal cerebral ischemia and reperfusion. Neural Regen Res. 2011;6(14):1086-1090.

67. Liu K, Li Z, Wu T, et al. Role of Rho Kinase in Microvascular Damage Following Cerebral Ischemia Reperfusion in Rats. Int. J. Mol. Sci. 2011; 12: 1222-1231.

68. Yang L, Zhang BS, Yin L, et al. Tanshinone IIA Prevented Brain Iron Dyshomeostasis in Cerebral Ischemic Rats. Cell Physiol Biochem, 2011; 27:23-30.

69. Sun L, Ai J, Wang N, et al. Cerebral Ischemia Elicits Aberration in Myocardium Contractile Function and Intracellular Calcium Handling. Cell Physiol Biochem 2010; 26:421-430.

70. Zhang F, Wu Y, Jia J et al. Pre-Ischemic Treadmill Training Induces Tolerance to Brain Ischemia: Involvement of Glutamate and ERK1/2. Molecules. 2010；15：5246-5257.

71. Wu Y, Ye XH, Guo PP, et al. Neuroprotective Effect of Lipoxin A4 Methy Ester in a Rat Model of Permanent Focal Cerebral Ischemia. J Mol Neurosci. 2010; 22:226-234.

72. Li LL, Zuo ZY. Isoflurane preconditioning improves short-term and long-term neurological outcome after focal brain ischemia in adult rats. J Neuroscience.2009; 164:497-506.

73. Xing BZ, Chen H, Zhang M. PhD. Ischemic Postconditioning Inhibits Apoptosis after Focal Cerebral Ischemia/Reperfusion Injury in the Rat. Stroke. 2008; 39:2362-2369.

74. Xing BZ, Chen H, Zhang M, et al. Ischemic post-conditioning protects brain and reduces inflammation in a rat model of focal cerebral ischemia/reperfusion. Journal of Neurochemistry. 2008; 105(5):1737-1745.